5 Litre Bioreactor Assembly (Cell Culture)

The following impellers are available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm diameter shaft, include M3 x 5 mm hex set-screw. The Vortex pumps the liquid “down”, creating a vortex in the liquid. The Scoping impeller is the opposite, and pumps the liquid “up”.

Air inlet pipe used to meet the oxygen demand of a culture. Allows a sterile gas stream to be sparged into the vessel. This is a “sintered frit” or “stone” type sparger which can be used when high gas flows might cause shear damage. The 316L sintered bead porous sparger generates very small bubbles, resulting in very efficient gas transfer. Supplied with 15 Micron sparge tip. Includes 10 mm Hex Nut, Washer and 8.5 x 2.5 mm Silicone O-ring.

Compatible with 5 Litre Vessel, 10 mm port.

316L stainless steel stand for 5 litre vessels. Includes adjustable braces to stabilize the lower portion of the vessel, and protective silicone rubber feet for the three legs. This prevents wobble and stray signals which may be transferred through the legs when placed in multiples on steel clad benchtops.

Compatible with 5 Litre Vessel & Accessories

Dished bottom bioreactor vessel, suitable for both fermentation and cell culture runs. Dished bottom ensures optimum mixing. Marked with approximate volume graduations, made from borosilicate glass, fully autoclavable.

Compatible with 5 litre vessel stand and single piece retainer ring.

Connects to the 5 litre vessel, forming a seal between the vessel and the stand. Single piece, 316L stainless steel.

Includes:

• 6 each M6 x 45 mm screws (attached)
• 3 each M4 x 16 mm flat head screws (ring to stand)
• 1 each Teflon/Silicone Gasket
• 1 each 177 x 4.0 mm Silicone O-ring

Compatible with 5 litre Vessel, Stand and Accessories

316L stainless steel electropolished headplate for 5-7 litre vessels. Threaded ports for a wide variety of accessory fittings. O-rings, fittings, etc. purchased separately.

Compatible with 5-7 litre Vessel, Stand and Accessories.

The following blind stoppers are available for the (M18 x 1.5 mm or G-3/4) threaded headplate ports and include Silicone O-ring.

O-ring Sizes:

G-3/4 = AS-023 Silicone

Compatible with 2 – 7 litre vessel, G3/4 port.

The following blind stoppers are available for the (M18 x 1.5 mm or G-3/4) threaded headplate ports and include Silicone O-ring.

O-ring Sizes:

M18 =  20 x 2.0

Compatible with 2 – 7 litre vessel, M18 x 1.5 port.

These blind stoppers are designed to plug unused ports into the headplate. The assemblies are fitted into the headplate from the inside of the reactor, leaving only a flat surface inside the reactor. Includes Hex Nut, Washer and Silicone O-ring.

O-ring sizes:

12 mm = AS-111

Compatible with 2 – 20 litre vessel, 12 mm port

These blind stoppers are designed to plug unused ports into the headplate. The assemblies are fitted into the headplate from the inside of the reactor, leaving only a flat surface inside the reactor. Includes Hex Nut, Washer and Silicone O-ring.

O-ring size:

10 mm = 8.5 x 2.5

Compatible with 2 – 20 litre vessel, 10mm port.

These are the fluted nuts used to attach the vessel headplate to the vessel base. Sold in packs of six each, sized for the 2 – 20 litre vessels.

Compatible with 2 – 20 litre Vessel base.

This height adjustable sample pipe assembly consists of an assembly holder for 6 mm tubes, and a 6 mm OD x 4 mm ID sample tube. With this device, the culture fluid can be sampled at any desired level. Includes 10 mm Hex Nut Washer and 8.5 x 2.5 mm Silicone O-ring.

Maximum insertion length:          213 mm (2 – 5 litre reactor)

Compatible with 2 – 5 litre vessel, 10 mm port.

The air overlay pipe can be used for either gas outlet or gas overlay. The latter use is for headspace aeration (separate from or in combination with sparging gas through the culture). Includes 10 mm Hex Nut, Washer and 8.5 x 2.5 mm Silicone O-ring.

Compatible with 2-20 Litre Vessels, 10 mm port.

Attaches to the tip of the Sparger Tube, this 316L stainless steel sintered tip is available in a variety of pore sizes. The 15 Micron pore frit is the standard size supplied with the sparger tube. Other sizes available upon request (see table). Includes 2.6 x 1.9 mm Silicone O-ring.

The thermowell allows you to insert a temperature probe in the reactor. Fill the thermometer pocket with water or silicone oil in order to improve thermal contact between the culture and the probe. Includes 10 mm Hex Nut, Washer and 8.5 x 2.5 mm Silicone O-ring.

Compatible with 2 – 5 litre vessel, 10 mm port.

Insertion length: 200 mm (for 2 – 5 litre reactor)

The septum holder is equipped with a silicone rubber septum and can be used as a universal addition port by piercing it with one or more needles. Includes 20 x 2.0 mm Silicone O-ring.

Compatible with 2 – 7 litre vessels, 18 mm port.

The media inlet triple port allows you to equip one M18 x 1.5 mm threaded port with three addition ports (e.g. for acid, alkali and anti-foam addition). This device can be used to expand the number of entries beyond the number of ports in the headplate. Includes 20 x 2.0 mm Silicone O-ring.

Compatible with 2-7 Litre Vessels, M18 x 1.5 port.

This housing fits into a G-3/4” threaded port and can accommodate a pH or Redox electrode, a Dissolved Oxygen sensor, a Cell Density probe, or other 12 mm OD tubes. All items that are held by this housing are at a fixed depth. Includes AS-111 internal and AS-023 external Silicone O-rings.

Compatible with 2 – 7 litre vessel, G3/4” port.

This housing fits into an M18 x 1.5 mm threaded port and can accommodate a pH or Redox electrode, a Dissolved Oxygen sensor, a Cell Density probe, or other 12 mm OD tubes. All items that are held by this housing are at a fixed depth. Includes AS-111 internal and 20 x 2.0 mm external Silicone O-rings.

Compatible with 2 – 7 litre vessel, M18 x 1.5 port.

Installs into the central M30 x 1.0 mm port of the 2 through 5 litre vessels. Includes 32 x 2.5 mm Silicone O-ring.

Material: 316L Stainless Steel

Shaft Diameter: 8 mm

Compatible with 2-5 Litre Vessel M30 Port

The following impellers are available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm diameter shaft, include M3 x 5 mm hex set-screw. The Vortex pumps the liquid “down”, creating a vortex in the liquid. The Scoping impeller is the opposite, and pumps the liquid “up”.

The following impeller is available for the agitator assemblies, both lipseal and magnetically coupled types. Fits onto the standard 8 mm diameter shaft, includes size M3 x 5 mm hex set-screw.

Compatible with 5-7 Litre Vessels, 8 mm Shaft.

This is used for heating the vessel contents and controlling to a set value, say 37 degrees Centigrade. Made of flexible silicone coated heating elements, it plugs into a standard 230 VAC temperature controller or vessel control system.

Standard 6’ cord length (Special lengths up to 15’ available on request).

Compatible with Standard 230 VAC Temp Controller.

Kit to self-rebuild the Lipseal Agitator Assembly.

Compatible with 2-20 Litre Small Agitator Assembly.

This height adjustable sample pipe assembly consists of an assembly holder for 6 mm tubes (B4-71002-2T9-M10), and

a 6 mm OD x 0.78 mm ID sample tube.

This sample pipe has a very small dead volume. The height adjustable sample pipe can be used with or without a sample screen. If it is used without a sample screen, the small dead volume of the pipe guarantees samples from the culture that are representative for the reactor.

If a sample screen is used at the end of this pipe, cell-free samples can be drawn from the culture. Sample screens are available in several pore sizes.

Compatible with 2 – 5 litre vessel, 10 mm port.

Installs into the central M30 x 1.0 mm threaded port of the 2 through 5 litre vessels. Includes 32 x 2.5 mm Silicone O-ring.

Material: 316L Stainless Steel

Shaft Diameter: 8 mm

Compatible with 2 – 5 litre vessel, M30 port

This device fits into the standard M18 x 1.5 threaded port and allows for cooling water to be circulated through it, chilling the contents of the vessel. Standard 6 mm tube fittings for attaching the cold-water tubing can be hooked up to either a standard bench water supply or to a recirculating chiller pump. Includes 20 x 2 mm Silicone O-ring.

Compatible with 2 – 5 litre vessel, 18 mm port.

Vessel operation at elevated temperature combined with aeration of the culture might cause too much evaporation during a run (increasing the nutrient concentration and decreasing volume); this can be prevented by using an air-outlet condenser. Includes Teflon spacer AS-111 Silicone O-ring and B472001-2T7-M18 Headplate fitting shown.

Standard 6 mm tube fittings for attaching the cold water tubing can be hooked up to either a standard bench water supply or to a recirculating chiller pump.

Compatible with 2 – 5 litre vessel, M18 x 1.5 port Sensor Fitting.